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1. (WO2015179701) ENGINEERED XYLOSE TRANSPORTERS WITH REDUCED GLUCOSE INHIBITION
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WHAT IS CLAIMED IS:

1. A recombinant xylose transporter protein comprising a xylose transporter motif sequence and a glucose mitigation mutation.

2. The recombinant xylose transporter protein of claim 1, wherein said xylose transporter motif sequence corresponds to amino acid residue positions 36, 37, 38, 39, 40, and 41 of Candida intermedia GXS1 protein.

3. The recombinant xylose transporter protein of claim 1 or claim 2, wherein said xylose transporter motif sequence is -G-G/F-X'-X^X^G-;

wherein,

X1 is D, C, G, H, I, L, or F;

X2 is A, D, C, E, G, H, or I; and

X3 is N, C, Q, F, G, L, M, S, T, or P.

4. The recombinant xylose transporter protein of one of claims 1-3, wherein said xylose transporter motif sequence is G-G-F-I-M-G- (SEQ ID NO: 107), -G-F-F-I-M-G-(SEQ ID NO: 108), -G-G-F-I-S-G- (SEQ ID NO: 109), -G-F-F-I-S-G- (SEQ ID NO: 110), -G-G-F-I-T-G- (SEQ ID NO: 111), -G-F-F-I-T-G- (SEQ ID NO: 112), -G-G-F-L-M-G- (SEQ ID NO: 113), -G-F-F-L-M-G- (SEQ ID NO: 114), -G-G-F-L-S-G- (SEQ ID NO: 115), -G-F-F-L-S-G-(SEQ ID NO: 116), -G-G-F-L-T-G- (SEQ ID NO: 117), -G-F-F-L-T-G- (SEQ ID NO: 118), -G-G-F-H-M-G- (SEQ ID NO: 119), -G-F-F-H-M-G- (SEQ ID NO: 120), -G-G-F-H-S-G- (SEQ ID NO: 121), -G-F-F-H-S-G- (SEQ ID NO: 122), -G-G-F-H-T-G- (SEQ ID NO: 123) or

-G-F-F-H-T-G- (SEQ ID NO: 124)..

5. The recombinant xylose transporter protein of any one of claims 1 to 4, wherein said xylose transporter motif sequence is G-G-F-I-M-G- (SEQ ID NO: 107), -G-F-F-I-M-G- (SEQ ID NO: 108), -G-G-F-I-S-G- (SEQ ID NO: 109), or -G-F-F-I-S-G- (SEQ ID

NO: 110).

6. The recombinant xylose transporter protein of any one of claims 1 to 5, wherein said xylose transporter motif sequence is -G-G-F-I-M-G- (SEQ ID NO: 107).

7. The recombinant xylose transporter protein of any one of claims 1 to 6, wherein said glucose mitigation mutation is within a protein domain corresponding to transmembrane 9 of Candida intermedia GXS1 protein.

8. The recombinant xylose transporter protein of any one of claims 1 to 7, wherein said glucose mitigation mutation is at a position corresponding to K155, N225, S354, A361, L407, or N446 of Candida intermedia GXS1 protein.

9. The recombinant xylose transporter protein of any one of claims 1 to 6, wherein said glucose mitigation mutation is within a protein domain corresponding to transmembrane 8 of Candida intermedia GXS1 protein.

10. The recombinant xylose transporter protein of any one of claims 1 to 6, or claim 9, wherein said glucose mitigation mutation is at a position corresponding to N326 of Candida intermedia GXS1 protein.

11. The recombinant xylose transporter protein of claim 10, wherein said glucose mitigation mutation is a N326H mutation.

12. The recombinant xylose transporter protein of claim 10, wherein said glucose mitigation mutation is a N326S mutation.

13. The recombinant xylose transporter protein of any one of claims 1 to 6, wherein said glucose mitigation mutation is within a protein domain corresponding to transmembrane 5 of Candida intermedia GXS1 protein.

14. The recombinant xylose transporter protein of any one of claims 1 to 6, or claim 13, wherein said glucose mitigation mutation is within a protein domain corresponding to residue 160-179 of Candida intermedia GXS1 protein.

15. The recombinant xylose transporter protein of any one of claims 1 to 6, 13 or 14, wherein said glucose mitigation mutation is at a position corresponding to T170 or 1171 of Candida intermedia GXS1 protein.

16. The recombinant xylose transporter protein of claim 15, wherein said glucose mitigation mutation is a T 170N mutation.

17. The recombinant xylose transporter protein of claim 15, wherein said glucose mitigation mutation is a 1171 F mutation.

18. The recombinant xylose transporter protein of one of claims 1-17 further comprising an amino acid deletion.

19. The recombinant xylose transporter protein of claim 18, wherein said deletion is within a protein domain corresponding to residue 497-522 of Candida intermedia GXS1 protein.

20. The recombinant xylose transporter protein of claim 18 or claim 19, wherein said deletion is at least 10 amino acids in length.

21. A recombinant yeast cell comprising a recombinant xylose transporter protein of any one of claims 1 to 20.

22. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 10% of the growth rate of said recombinant yeast cell in a xylose growth media.

23. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 20% of the growth rate of said recombinant yeast cell in a xylose growth media.

24. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 30% of the growth rate of said recombinant yeast cell in a xylose growth media.

25. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 40% of the growth rate of said recombinant yeast cell in a xylose growth media.

26. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 50% of the growth rate of said recombinant yeast cell in a xylose growth media.

27. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 60% of the growth rate of said recombinant yeast cell in a xylose growth media.

28. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 70% of the growth rate of said recombinant yeast cell in a xylose growth media.

29. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 80% of the growth rate of said recombinant yeast cell in a xylose growth media.

30. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 90% of the growth rate of said recombinant yeast cell in a xylose growth media.

31. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 100% of the growth rate of said recombinant yeast cell in a xylose growth media.

32. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 110% of the growth rate of said recombinant yeast cell in a xylose growth media.

33. The recombinant yeast cell of claim 21 , wherein the growth rate of said recombinant yeast cell in a xylose-glucose growth media is at least about 120% of the growth rate of said recombinant yeast cell in a xylose growth media.

34. The recombinant yeast cell of any one of claims 21 to 33, wherein said xylose-glucose growth media comprises about 0.05 g/L to about 20 g/L glucose.

35. The recombinant yeast cell of any one of claims 21 to 34, wherein said xylose-glucose growth media comprises about 2.5 g/L glucose.

36. The recombinant yeast cell of any one of claims 21 to 35, wherein said xylose-glucose growth media comprises about 5 g/L glucose.

37. The recombinant yeast cell of any one of claims 21 to 36, wherein said xylose-glucose growth media comprises about 10 g/L glucose.

38. The recombinant yeast cell of any one of claims 21 to 37, wherein said xylose-glucose growth media comprises about 20 g/L glucose.

39. The recombinant yeast cell of any one of claims 21 to 38, wherein said xylose-glucose growth media comprises about 0.05 g/L to about 300 g/L xylose.

40. The recombinant yeast cell of any one of claims 21 to 39, wherein said xylose growth media comprises about 0.05 g/L to about 300 g/L xylose.

41. The recombinant yeast cell of any one of claims 21 to 40, wherein said xylose growth media comprises about 20 g/L xylose.

42. A method of transporting xylose into a recombinant yeast cell, said method comprising:

i) contacting a recombinant yeast cell with a xylose compound, wherein said recombinant yeast cell comprises a recombinant xylose transporter protein, said recombinant xylose transporter protein comprising a xylose transporter motif sequence and a glucose mitigation mutation ; and

ii) allowing said recombinant xylose transporter protein to transport said xylose compound into said recombinant yeast cell.

43. The method of claim 42, wherein said xylose transporter motif sequence corresponds to amino acid residue positions 36, 37, 38, 39, 40, and 41 of Candida intermedia GXS1 protein.

44. The method of claim 42 or claim 43, wherein said xylose transporter motif sequence is

wherein,

X1 is D, C, G, H, I, L, or F;

X2 is A, D, C, E, G, H, or I; and

X3 is N, C, Q, F, G, L, M, S, T, or P.

45. The method of one of claims 42-44, wherein said xylose transporter motif sequence is -G-G-F-I-M-G- (SEQ ID NO: 107), -G-F-F-I-M-G- (SEQ ID NO: 108), -G-G-F-I-S-G- (SEQ ID NO: 109), -G-F-F-I-S-G- (SEQ ID NO: 110), -G-G-F-I-T-G- (SEQ ID NO: 111), -G-F-F-I-T-G- (SEQ ID NO: 112), -G-G-F-L-M-G- (SEQ ID NO: 113), -G-F-F-L-M-G- (SEQ ID NO: 114), -G-G-F-L-S-G- (SEQ ID NO: 115), -G-F-F-L-S-G- (SEQ ID NO: 116), -G-G-F-L-T-G-(SEQ ID NO: 117), -G-F-F-L-T-G- (SEQ ID NO: 118), -G-G-F-H-M-G- (SEQ ID NO: 119), -G-F-F-H-M-G- (SEQ ID NO: 120), -G-G-F-H-S-G- (SEQ ID NO: 121), -G-F-F-H-S-G- (SEQ ID NO: 122), -G-G-F-H-T-G- (SEQ ID NO: 123) or -G-F-F-H-T-G- (SEQ ID NO: 124).

46. The method of any one of claims 42 to 45, wherein said xylose transporter motif sequence is -G-G-F-I-M-G- (SEQ ID NO : 107), -G-F-F-I-M-G- (SEQ ID NO : 108), -G-G-F-I-S-G- (SEQ ID NO: 109), or -G-F-F-I-S-G- (SEQ ID NO: 110).

47. The method of any one of claims 42 to 46, wherein said xylose transporter motif sequence is -G-G-F-I-M-G- (SEQ ID NO: 107).

48. The method of any one of claims 42 to 47, wherein said glucose mitigation mutation is within a protein domain corresponding to transmembrane 9 of Candida intermedia GXS1 protein.

49. The method of any one of claims 42 to 48, wherein said glucose mitigation mutation is at a position corresponding to K155, N225, S354, A361, L407, or N446 of Candida intermedia GXS1 protein.

50. The method of any one of claims 42 to 47, wherein said glucose mitigation mutation is within a protein domain corresponding to transmembrane 8 of Candida intermedia GXS1 protein.

51. The method of any one of claims 42 to 47, or claim 50, wherein said glucose mitigation mutation is at a position corresponding N326 of Candida intermedia GXS1 protein.

52. The method of claim 51 , wherein said glucose mitigation mutation is a N326H mutation.

53. The method of claim 51 , wherein said glucose mitigation mutation is a N326S mutation.

54. The method of any one of claims 42 to 47, wherein said glucose mitigation mutation is within a protein domain corresponding to transmembrane 5 of Candida intermedia GXS1 protein.

55. The method of one of claims 42 to 47, or claim 54, wherein said glucose mitigation mutation is within a protein domain corresponding to residue 160-179 of Candida intermedia GXS1 protein.

56. The method of claim 54 or 55, wherein said glucose mitigation mutation is at a position corresponding to T170 or 1171 of Candida intermedia GXS1 protein.

57. The method of claim 56, wherein said glucose mitigation mutation is a T170N mutation.

58. The method of claim 56, wherein said glucose mitigation mutation is a II 7 IF mutation.

59. The method of any one of claims 42 to 58, further comprising an amino acid deletion.

60. The method of claim 59, wherein said deletion is within a protein domain corresponding to residue 497-522 of Candida intermedia GXS1 protein.

61. The method of any one of claims 42 to 53 , wherein said recombinant yeast cell metabolizes said xylose compound.

62. The method of any one of claims 42 to 61, wherein said recombinant yeast cell converts said xylose compound to a biofuel.

63. The method of any one of claims 42 to 62, wherein said xylose compound forms part of lignocellulosic biomass, hemicellulose, or xylan.

64. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 10% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

65. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 20% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

66. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 30% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

67. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 40% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

68. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 50% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

69. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 60% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

70. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 70% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

71. The method of any one of claims 42 to 63 , wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 80% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

72. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 90% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

73. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 100% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

74. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 1 10% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

75. The method of any one of claims 42 to 63, wherein said recombinant xylose transporter transports said xylose compound into said yeast in a xylose-glucose growth media at a rate at least about 120% of the rate said recombinant xylose transporter transports said xylose compound into said yeast in a xylose growth media.

76. The method of any one of claims 64 to 75, wherein said xylose-glucose growth media comprises about 0.05 g/L to about 20 g/L glucose.

77. The method of any one of claims 64 to 76, wherein said xylose-glucose growth media comprises about 2.5 g/L glucose.

78. The method 1 of any one of claims 64 to 77, wherein said xylose-glucose growth media comprises about 5 g/L glucose.

79. The method of any one of claims 64 to 78, wherein said xylose-glucose growth media comprises about 10 g/L glucose.

80. The method of any one of claims 64 to 79, wherein said xylose-glucose growth media comprises about 20 g/L glucose.

81. The method of any one of claims 64 to 80, wherein said xylose-glucose growth media comprises about 0.05 g/L to about 300 g/L xylose.

82. The method of any one of claims 64 to 81, wherein said xylose growth media comprises about 0.05 g/L to about 300 g/L xylose.

83. The method of any one of claims 64 to 82, wherein said xylose growth media comprises about 20 g/L xylose.

84. The method of any one of claims 42 to 83, wherein said recombinant xylose transporter protein transports said xylose compound into said recombinant yeast cell in a xylose-glucose growth media growth media at a rate of at least 5 nmol min-1 gDCW-1.

85. A nucleic acid encoding the recombinant xylose transporter protein of one of claims 1 to 21.