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1. (WO2005045425) METHOD AND APPARATUS FOR DETERMINING CELL VIABILITY

Pub. No.:    WO/2005/045425    International Application No.:    PCT/ES2004/000492
Publication Date: Fri May 20 01:59:59 CEST 2005 International Filing Date: Fri Nov 05 00:59:59 CET 2004
IPC: G01N 1/30
G01N 33/483
Applicants: UNIVERSIDAD DE BARCELONA
PUIGNOU GARCIA, Lluis
SANZ JURADO, Ramsés
GALCERAN HUGUET, M. Teresa
Inventors: PUIGNOU GARCIA, Lluis
SANZ JURADO, Ramsés
GALCERAN HUGUET, M. Teresa
Title: METHOD AND APPARATUS FOR DETERMINING CELL VIABILITY
Abstract:
The invention relates to a method of determining cell viability and to an apparatus that has been specially designed for said purpose. The inventive method, which involves the use of gravitational field-flow fractionation (GrFFF) with fluorescence detection, is suitable for commercial yeast strains. The effectiveness of the method can be demonstrated by comparing the cell viability results obtained using GrFFF with those obtained using flow cytometry and Coulter counter techniques. Said method offers good linearity, repeatability and a sufficiently-low detection limit for the detection of 2 x 104 non-viable cells. In addition, GrFFF can be easily implemented in a standard liquid-chromatography system. In this way, the simplicity and low cost of the apparatus make it a valuable alternative to the flow-cytometry- and Coulter-counter-based devices currently on the market.