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1. US20120021424 - Device and method for thermal cycling

Anmerkung: Text basiert auf automatischer optischer Zeichenerkennung (OCR). Verwenden Sie bitte aus rechtlichen Gründen die PDF-Version.

[ EN ]

Claims

1. A method of performing nucleic acid amplification comprising the steps of:
loading a plurality of sample wells with a plurality of biological samples;
providing a device configured to thermally cycle a sample block assembly, the sample block assembly comprising a sample block and a heat sink;
placing the loaded plurality of sample wells onto a sample well receiving region of a sample well holder;
moving the sample well holder and loaded plurality of sample wells into the device until the plurality of sample wells is aligned between the sample block assembly positioned beneath the plurality of sample wells and the sample well holder and a stationary optical detection system;
moving the sample block assembly from a first position permitting the sample well holder to align the plurality of sample wells with the sample block assembly to a second position permitting the sample block to contact the plurality of sample wells;
thermally cycling the sample block assembly; and
detecting the plurality of biological samples while thermal cycling the sample block assembly.
2. The method of claim 1, wherein the step of detecting includes using a CCD camera.
3. The method of claim 1, wherein the plurality of sample wells is a 96-well sample tray.
4. The method of claim 1, wherein the plurality of sample wells is a 384-well sample tray.
5. The method of claim 1, wherein the plurality of sample wells is a microcard.
6. A method of performing nucleic acid amplification comprising the steps of:
loading a plurality of sample wells with a plurality of biological samples;
providing a device configured to thermally cycle a sample block assembly, the thermal block assembly comprising a thermal block and a heat sink;
placing the loaded plurality of sample wells onto a sample well receiving region of a sample well holder;
moving the sample well holder with the loaded plurality of sample wells into the device until the plurality of sample wells is aligned between the sample block assembly positioned beneath the plurality of sample wells and the sample well holder and a stationary optical detection system;
providing movement between the sample well holder with the plurality of sample wells and the sample block assembly, thereby providing contact between the plurality of sample wells and the sample block assembly;
thermally cycling the sample block assembly; and
detecting the plurality of biological samples while thermal cycling the sample block assembly.
7. The method of claim 6, wherein the optical detection system is positioned above the sample block assembly.
8. The method of claim 7, wherein the optical detection system is configured to reduce misalignment of optical components.
9. The method of claim 7, wherein the sample well holder and sample wells are dimensioned so that they are capable of passing between the optical detection system and the sample block assembly.
10. The method of claim 9, wherein the sample well holder and sample wells are dimensioned so that they are capable of passing between the optical detection system and the sample block assembly without interference.
11. The method of claim 6, wherein the sample well receiving region is generally rectangular in shape.
12. The method of claim 6, wherein the sample well receiving region supports the plurality of sample wells.
13. The method of claim 6, wherein the plurality of sample wells is a 96-well sample tray.
14. The method of claim 6, wherein the plurality of sample wells is a 384-well sample tray.
15. A method of performing nucleic acid amplification comprising the steps of:
providing a sample well tray comprising of a plurality of sample wells;
loading the sample well tray with a plurality of biological samples, wherein the sample well tray has a top surface and a bottom surface;
providing a device configured to thermally cycle a sample block assembly, the sample block assembly comprising a heat sink and a sample block having a top surface comprising a plurality of recesses arranged to correspond to the sample wells of the sample well tray;
placing the loaded sample well tray onto a sample well receiving region of a sample well holder;
moving the sample well holder with the loaded sample well tray into the device until the sample well tray is aligned between the plurality of recesses of the sample block positioned beneath the sample well tray and the sample well holder and a stationary optical detection system;
contacting the top surface of the sample block with the bottom surface of the sample well tray so that the sample wells are seated against the sample block;
thermally cycling the sample block; and
detecting the plurality of biological samples while thermal cycling the sample block.