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1. US20110281259 - Method and apparatus for the simultaneous, automated decomposition of a plurality of biological samples

Anmerkung: Text basiert auf automatischer optischer Zeichenerkennung (OCR). Verwenden Sie bitte aus rechtlichen Gründen die PDF-Version.

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Claims

1. Method for simultaneously disrupting a plurality of biological samples, comprising the steps of:
the samples are filled into a plurality of non-metallic containers,
at least one movable hard body is filled into each container,
the containers are inserted into an adapter having casings of metal disposed in a circle about a central axis of the adapter wherein the adapter is cooled to between −20° C. and −80° C., and wherein the adapter is not actively cooled during reciprocation,
the adapter, with the sealed containers located therein, is connected to an apparatus that automatically reciprocates the adapter.
2. Method according to claim 1, wherein the samples are first washed and then frozen with liquid nitrogen or chemically preserved, and the samples thus prepared are filled into the container.
3. Method according to claim 1, wherein the containers, samples and adapter are first cooled to between −20° C. and −80° C., and the sample is subsequently disrupted by reciprocating the adapter.
4. Method according to claim 1, wherein the at least one moveable hard body comprises metal or glass.
5. Method according to claim 1, wherein a lysis buffer is filled into the containers prior to disruption.
6. Method according to claim 1, wherein the adapter is reciprocated at a frequency of at least 10 Hz.
7. Method according to claim 1, wherein the weight of a sample filled into a container is no more than 50 mg.
8. Method according to claim 1, wherein the containers comprise plastic.
9. Device for carrying out a method according to claim 1, comprising an adapter comprising plastic, with casings comprising metal for accommodating non-metallic containers.
10. Device according to claim 9, wherein the adapter is connected detachably, to an apparatus that is capable of automatically reciprocating the adapter parallel to the casings.
11. Device according to claim 10, wherein the apparatus is configured such that it is capable of reciprocating the adapter at a frequency of at least 10 Hz.
12. Device according to claim 9, with containers which are inserted into the casings and in which a biological sample and, additionally, a moveable hard body is located.
13. Device according to claim 9, with an internal casing diameter of the metallic casings of no more than 16 mm.
14. Device according to claim 9, with at least six metallic casings for accommodating containers.
15. Device according to claim 9, wherein the casings are retained by an insert that is removable from the adapter.
16. Device according to claim 9 with casings which, in a top view, are disposed in the shape of a ring.
17. Device according to claim 9, with a lid that can be firmly connected to the adapter.
18. The method of claim 1, wherein said adapter is not actively cooled during reciprocation.
19. The method of claim 5, wherein said lysis buffer comprises a buffer comprising complexing agents and surfactants for disrupting DNA, or a buffer comprising a chaotropic agent for disrupting RNA.
20. The method of claim 1, wherein said adapter consists of plastic.