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1. (WO2019032069) A BIOMIMETIC ARTIFICIAL BLOOD VESSEL AND A PRODUCTION METHOD THEREOF
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CLAIMS

1. A method for a production of biomimetic artificial blood vessel (100) characterized in that said method comprises the steps of; - preparation of ethyl cellose solution (101) at a certain concentration by dissolving biocompatible and low toxicity natural ethyl cellulose polymer in at least one organic solvent,

Preparation of polycapro lactone solution (102) at a certain concentration by dissolving the synthetic polycaprolactone polymer in at least one organic solvent, Mixing the ethylcellulose solution and the polycaprolactone solution at a certain ratio (103)

Addition of collagen dissolved in at least one organic solvent into the mixture comprising ethyl cellulose and polycaprolactone solution (104), at a certain ratio Stirring the mixture comprising ethyl cellulose, polycaprolactone and collagen to have a homogenous solution (105),

- Subjecting the homogenous solution comprising ethyl cellulose, polycaprolactone and collagen to the electrospinning process (106) for a while in an electrospinning machine that comprise at least one needle and a movable, cylindrical collector via adjusting parameters of voltage, distance between the needle and the collector and flow rate that effect the nanofiber diameter and morphology,

- Obtaining the hollow nano-composite structure scaffold (107) via subjecting the mixture to electrospinning (106) and then collecting the nano fibers formed by movement of the polymers with the static electricity to form nanofibers on the cylindrical collector and

Obtaining of the artificial blood vessel (108) via drying of the obtained structure scaffold.

2. An artificial blood vessel production method (100) according to Claim 1, characterized in that the step (101) the ethyl cellulose is dissolved in the organic solvent such that there is 1% of ethyl cellulose in the solution by weight.

3. An artificial blood vessel production method (100) according to claim 1 or 2, characterized in that the ethyl cellulose solution (101) is prepared by addition of ethyl cellulose to a solution comprising dimethylacetamide (DMA) and tetrahydrofuran (THF) in a ratio of 60:40 by weight.

4. An artificial blood vessel production method (lOO)according to any one of the preceding claims, characterized in that in step (102) the polycaprolactone solution is prepared as a 10% by weight polycaprolactone polymer in the solvent.

5. An artificial blood vessel production method (100) according to any one of the preceding claims, characterized in that in step (102) polycaprolactone solution is prepared by adding polycaprolactone polymer to the solvent comprising dimethylacetamide (DMA) and tetrahydrofuran (THF) in a ratio of 60:40 by weight.

6. An artificial blood vessel production method (100) according to any one of the preceding claims, characterized in that in step (103) ethyl cellulose solution and the polycaprolactone solution is mixed by 50:50 by weight.

7. An artificial blood vessel production method (100) according to any one of the preceding claims, characterized in that in step (104) collagen dissolved in acetic acid is added to ethyl cellulose and polycaprolactone solution.

8. An artificial blood vessel production method (100) according to any one of the preceding claims, characterized in that in step (104) a solution of collagen is added to the solution comprising ethyl cellulose and polycaprolactone, such that the mixture comprise 0.1% of collagen by weight (104)

9. An artificial blood vessel production method (100) according to any one of the preceding claims, characterized in that in step (105) a mixture comprising ethyl cellulose, polycaprolactone and collagen are mixed in a magnetic stirrer for 10 minutes.

10. An artificial blood vessel manufacturing method (100) according to any one of the preceding claims, characterized in that the mixture comprising ethyl cellulose, polycaprolactone and collagen is transferred to an injector and loaded to the pump section of an electrospinning machine, which leads to progression of a predetermined amount of polymer at a predetermined time interval.

11. An artificial blood vessel production method (100) according to any one of the above claims, characterized in that the mixture containing ethyl cellulose, polycaprolactone and collagen is subjected to electrospinning treatment at a flow rate of 1.75 ml / hr.

12. An artificial blood vessel production method (100) according to any one of the preceding claims, characterized in that the mixture comprising ethyl cellulose, polycaprolactone and collagen is subjected to electrospinning (106) at a voltage value of 36 kV.

13. An artificial blood vessel production method (100) according to any one of the above claims, characterized in that the mixture containing ethyl cellulose, polycaprolactone and collagen is subjected to electrospinning procedure for a period of 3 to 5 hours (106).

14. An artificial blood vessel production method (100) according to claim 14, characterized in that the mixture containing ethyl cellulose, polycaprolactone and collagen is subjected to electrospinning procedure for a period of 4 hours.

15. An artificial blood vessel production method (100) according to any of the preceding claims, characterized in that the the electrospinning procedure (106) is carried out with a cylindrical structured collector having a diameter of 0.5 cm.

16. An artificial blood vessel production method (100) according to any one of the preceding claims, characterized in that the artificial blood vessel is obtained (108) by drying the nanocomposite structure at an oven temperature of 23 to 50 ° C.

17. An artificial blood vessel production method (100) according to claim 16, characterized in that the step of obtaining the artificial blood vessel (108) by drying the nano composite scaffold in an oven is performed at 35 °C.

18. An artificial blood vessel production method (100) according to claim 16 or 17, characterized in that the step of obtaining artificial blood vessel (108) is performed by drying the nano composite scaffold in the oven for 3 to 8 hours.

19. An artificial blood vessel production method (100) according to claim 18, characterized in that the step (108) of obtaining the artificial blood vessel is performed by drying the nano composite scaffold for 4 hours in the oven.

20. A biomimetic artificial blood vessel comprising ethyl cellulose, polycaprolactone and collagen obtained by a method according to any one of claims 1 to 19.