البحث في مجموعات البراءات الوطنية والدولية

1. (WO2018135027) METHOD FOR PRODUCING HEPAROSAN COMPOUND HAVING ISOMERIZED HEXULONIC ACID RESIDUE

Pub. No.:    WO/2018/135027    International Application No.:    PCT/JP2017/030998
Publication Date: Fri Jul 27 01:59:59 CEST 2018 International Filing Date: Wed Aug 30 01:59:59 CEST 2017
IPC: C12P 19/26
C12N 9/90
C12N 15/09
Applicants: AJINOMOTO CO., INC.
味の素株式会社
Inventors: TSUJI, Chihiro
辻 ちひろ
MIHARA, Yasuhiro
三原 康博
NAKANO, Shogo
中野 祥吾
MOTOYAMA, Tomoharu
本山 智晴
ITO, Souhei
伊藤 創平
Title: METHOD FOR PRODUCING HEPAROSAN COMPOUND HAVING ISOMERIZED HEXULONIC ACID RESIDUE
Abstract:
The present invention provides: a method for producing a heparosan compound having an isomerized hexulonic acid residue, which has improved C5-epimerization efficiency; and a method for producing heparan sulfate. More specifically, the present invention provides a method for producing a heparosan compound having an isomerized hexulonic acid residue, said method comprising producing the heparosan compound having an isomerized hexulonic acid residue from a heparosan compound in the presence of a protein selected from the group consisting of the following proteins (A) to (F): (A) a protein which contains the amino acid sequence represented by SEQ ID NO: 2; (B) a protein which contains an amino acid sequence having a 90% or higher homology with the amino acid sequence represented by SEQ ID NO: 2 and has a D-glucuronyl C5-epimerase activity; (C) a protein which contains an amino acid sequence having the deletion, substitution, addition or insertion of one or several amino acid residues in the amino acid sequence represented by SEQ ID NO: 2 and has a D-glucuronyl C5-epimerase activity; (D) a protein which contains the amino acid sequence represented by SEQ ID NO: 5; (E) a protein which contains an amino acid sequence having a 90% or higher homology with the amino acid sequence represented by SEQ ID NO: 5 and has a D-glucuronyl C5-epimerase activity; and (F) a protein which contains an amino acid sequence having the deletion, substitution, addition or insertion of one or several amino acid residues in the amino acid sequence represented by SEQ ID NO: 5 and has a D-glucuronyl C5-epimerase activity.