البحث في مجموعات البراءات الوطنية والدولية

1. (WO1989012679) METHOD FOR PRODUCING THERMALLY STABLE ALPHA-AMYLASE

Pub. No.:    WO/1989/012679    International Application No.:    PCT/US1989/002683
Publication Date: Fri Dec 29 00:59:59 CET 1989 International Filing Date: Tue Jun 20 01:59:59 CEST 1989
IPC: C12N 9/28
Applicants: GREAT LAKES CHEMICAL CORPORATION
Inventors: CHEN, James, C.
LAMM, William, R.
RACINE, Francis, M.
TURNER, Hal, R.
Title: METHOD FOR PRODUCING THERMALLY STABLE ALPHA-AMYLASE
Abstract:
A process for the production of thermally stable alpha-amylase comprising the steps of: cultivating B. licheniformis ATCC 53,722 in a nutrient medium which contains as a primary carbon source a carbohydrate which normally represses alpha-amylase synthesis, selected from the group consisting of dextrose, starch hydrolysate, glycerol, xylose, mannose, sucrose and mixtures thereof; feeding said primary carbon source to said medium during cultivation; maintaining the level of carbon dioxide evolution from the medium during cultivation between about 4.5 % and 6.5 % of the off-gas when the air flow is 0.5 to 1.0 vvm by controlling the rate at which the primary carbon source is fed to said medium during cultivation; and maintaining the pH of the medium between 6.5 and 7.0.