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1. (EP2431461) Lipase congeners with enhanced substrate access
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Claims

1. Lipase enzyme which has been modified by the incorporation of one or more non-canonical amino acids, wherein said modified lipase enzyme has an increased enzymatic activity compared to the unmodified lipase enzyme in an aqueous phase.
  2. Lipase enzyme of claim 1, wherein said enzyme is derived from a thermophilic bacterium.
  3. Lipase enzyme of claim 1 or 2, wherein said enzyme is derived from Thermoanaerobacter thermohydrosulfuricus.
  4. Lipase enzyme of any of claims 1-3, wherein said enzyme comprises or consists of the sequence of SEQ ID NO:1 or an amino acid sequence which is at least 70% identical thereto.
  5. Lipase enzyme of any of claims 1-4, wherein said modified enzyme has an activity that is increased at least 2-fold compared to the unmodified enzyme.
  6. Lipase enzyme of any of claims 1-5, wherein said enzyme has been modified by incorporating one or more non-canonical amino acids in the lid region of the lipase enzyme.
  7. Lipase enzyme of claim 6, wherein said enzyme has been modified to increase the overall hydrophobicity of the lid region.
  8. Lipase enzyme of any of claim 6 or 7, wherein said enzyme has been modified by replacement of one or more native methionine residues in the lid region.
  9. Lipase enzyme of claim 8, wherein the one or more methionine residues have been replaced by a hydrophobic non-canonical amino acid.
  10. Lipase enzyme of claim 9, wherein the one or more methionine residues have been replaced by norleucine (Nle).
  11. Lipase enzyme of any of claims 1-5, wherein said enzyme has been modified by replacement of one or more native phenylalanine residues.
  12. Lipase enzyme of claim 11, wherein the one or more phenylalanine residues have been replaced by a fluorinated non-canonical amino acid.
  13. Lipase enzyme of claim 12, wherein the one or more phenylalanine residues have been replaced by meta-fluorophenylalanine (mFF) or para-fluorophenylalanine (pFF).
  14. Use of the lipase enzyme of any of claims 1-10 as an additive in soaps and/or detergents.
  15. Use of the lipase enzyme of any of claims 1-10 in the food industry.
  16. Soap and/or detergent composition comprising the lipase enzyme of any of claims 1-13.