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37. (WO2011138006) SENSORS FOR THE DETECTION OF INTRACELLULAR METABOLITES
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Latest bibliographic data on file with the International Bureau   
Pub. No.:    WO/2011/138006    International Application No.:    PCT/EP2011/002196
Publication Date: 10.11.2011 International Filing Date: 03.05.2011
IPC:
C12N 15/09 (2006.01), C12N 15/63 (2006.01), C12P 13/08 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13
Preparation of nitrogen-containing organic compounds
04
Alpha- or beta-amino acids
08
Lysine; Diaminopimelic acid; Threonine; Valine
Applicants: FORSCHUNGSZENTRUM JÜLICH GMBH [DE/DE]; Leo-Brandt-Strasse 52428 Jülich (DE) (For All Designated States Except US).
EGGELING, Lothar [DE/DE]; (DE) (For US Only).
BOTT, Michael [DE/DE]; (DE) (For US Only).
BINDER, Stephan [DE/DE]; (DE) (For US Only).
FRUNZKE, Julia [DE/DE]; (DE) (For US Only).
MUSTAFI, Nurije [DE/DE]; (DE) (For US Only)
Inventors: EGGELING, Lothar; (DE).
BOTT, Michael; (DE).
BINDER, Stephan; (DE).
FRUNZKE, Julia; (DE).
MUSTAFI, Nurije; (DE)
Agent: HERZOG, Martin; Herzog Fiesser & Partner Immermannstrasse 40 40210 Düsseldorf (DE)
Priority Data:
10 2010 019 059.4 03.05.2010 DE
Title (DE) SENSOREN ZUR INTRAZELLULÄREN METABOLIT-DETEKTION
(EN) SENSORS FOR THE DETECTION OF INTRACELLULAR METABOLITES
(FR) CAPTEURS POUR LA DÉTECTION DE MÉTABOLITES INTRACELLULAIRES
Abstract: front page image
(DE)Die vorliegende Erfindung betrifft eine gegenüber ihrem Wildtyp gentechnisch veränderte Zelle, die eine für ein Autofluoreszenzprotein kodierende Gensequenz umfasst, wobei die Expression des Autofluoreszenzproteins von der intrazellulären Konzentration eines bestimmten Metaboliten abhängig ist. Die vorliegende Erfindung betrifft auch ein Verfahren zum Identifizieren einer Zelle mit erhöhter intrazellulärer Konzentration eines bestimmten Metaboliten, ein Verfahren zur Herstellung einer gegenüber ihrem Wildtyp gentechnisch veränderten Zelle mit optimierter Produktion eines bestimmten Metaboliten, eine durch dieses Verfahren erhaltene Zelle, ein Verfahren zur Herstellung von Metaboliten sowie ein Verfahren zur Herstellung einer Mischung.
(EN)The present invention relates to a cell which has been modified recombinantly over its wild type and which comprises a gene sequence coding for an autofluorescent protein, the expression of the autofluorescent protein being dependent on the intracellular concentration of a specific metabolite. The present invention also relates to a method of identifying a cell with an increased intracellular concentration of a specific metabolite, to a method of generating a cell which has been modified recombinantly over its wild type and which demonstrates optimized production of a specific metabolite, to a cell obtained by this method, to a process for the production of metabolites, and to a process for the preparation of a mixture.
(FR)L'invention concerne une cellule génétiquement modifiée par rapport à son type sauvage, qui comprend une séquence de gènes codant pour une protéine autofluorescente, l'expression de la protéine autofluorescente dépendant de la concentration intracellulaire d'un métabolite déterminé. La présente invention concerne également un procédé d'identification d'une cellule contenant une concentration intracellulaire accrue d'un métabolite déterminé, un procédé de préparation d'une cellule génétiquement modifiée par rapport à son type sauvage présentant une production optimisée d'un métabolite déterminé, une cellule obtenue par ce procédé, un procédé de préparation de métabolites ainsi qu'un procédé de préparation d'un mélange.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Org. (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (EAPO) (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (OAPI) (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: German (DE)
Filing Language: German (DE)