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1. (WO1992020788) A PROCESS FOR PROVIDING ENHANCED YIELD AND SIMPLIFIED ISOLATION OF HYDROPHOBIC ENZYMES FROM CULTURE MEDIA
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1992/020788    International Application No.:    PCT/US1992/003127
Publication Date: 26.11.1992 International Filing Date: 17.04.1992
IPC:
C12N 9/00 (2006.01), C12N 9/20 (2006.01)
Applicants: OLIN CORPORATION [US/US]; 350 Knotter Drive, P.O. Box 586, Cheshire, CT 06410-0586 (US)
Inventors: HOLMES, Paul, E.; (US)
Agent: BURDICK, Bruce, E.; Olin Corporation, 350 Knotter Drive, P.O. Box 586, Cheshire, CT 06410-0586 (US)
Priority Data:
703,317 20.05.1991 US
Title (EN) A PROCESS FOR PROVIDING ENHANCED YIELD AND SIMPLIFIED ISOLATION OF HYDROPHOBIC ENZYMES FROM CULTURE MEDIA
(FR) PROCEDE DESTINE A AMELIORER LE RENDEMENT ET A SIMPLIFIER LA SEPARATION D'ENZYMES HYDROPHOBES DE MILIEUX DE CULTURE
Abstract: front page image
(EN)This invention is directed to a process for providing enhanced yield and simplified isolation of hydrophobic enzymes from a culture medium. The process comprises the steps of: (a) culturing a hydrophobic enzyme-secreting microorganism in an aqueous culture medium comprising a yield-enhancing effective amount of a nonionic surfactant having a cloud point of less thant 40 °C for a time sufficient to provide enhanced secretion of said enzyme from said microorganism, (b) removing said microorganism from said culture medium to provide a microorganism-free solution, (c) heating said microorganism-free solution to a temperature above said cloud point to cause phase separation of said culture medium into an aqueous phase, and a non-aqueous phase containing said nonionic surfactant and said yield-enhanced hydrophobic enzyme, and (d) isolating said non-aqueous phase containing said nonionic surfactant and said yield-enhanced hydrophobic enzyme.
(FR)Procédé destiné à améliorer le rendement et à simplifier la séparation d'enzymes hydrophobes d'un milieu de culture. Ledit procédé consiste (a) à cultiver un micro-organisme sécrétant un enzyme hydrophobe dans un milieu de culture aqueux comprenant une quantité efficace d'un tensio-actif non ionique, destinée à améliorer le rendement, possédant une température critique de dissolution inférieure à 40 °C, pendant un laps de temps suffisant pour que ledit micro-organisme augmente sa sécrétion dudit enzyme, (b) à éliminer ledit micro-organisme dudit milieu de culture afin d'obtenir une solution exempte de micro-organismes, (c) à chauffer ladite solution exempte de micro-organismes à une température supérieure à ladite température critique de dissolution pour provoquer la séparation en phase dudit milieu de culture en une phase aqueuse et en une phase non aqueuse contenant ledit tensio-actif non ionique et ledit enzyme hydrophobe à rendement amélioré et (d) à isoler ladite phase non aqueuse contenant le tensio-actif non ionique et l'enzyme hydrophobe à rendement amélioré.
Designated States: AU, BB, BG, BR, CA, FI, HU, JP, KP, KR, LK, MG, MW, NO, PL, RO, RU, SD.
European Patent Office (EPO) (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, MC, NL, SE)
African Intellectual Property Organization (OAPI) (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)